Protocols thatmove the needle.

Every protocol is graded by evidence level, linked to your biomarker results, and updated as new research emerges. No wellness fluff — only clinically-referenced interventions.

3
Strong
RCTs + Meta-analyses
2
Emerging
Pilot studies + mechanistic
1
Clinical
Physician-supervised
BeginnerStrong Evidence
PRO-001

NAD+ / NMN Protocol

Cellular Energy

Restore the fuel your mitochondria need to function optimally.

NMN is a direct precursor to NAD+, which declines ~50% by age 50. A 2023 RCT (n=80) showed 300–900mg NMN daily increased blood NAD+ significantly with no safety issues in 60 days. A 2024 study found 250mg/day improved walking speed and sleep quality in older adults after 12 weeks.

Duration
60 days minimum
Frequency
Daily, morning
Evidence
Strong
Yoshino et al., 2023 Cell Metabolism RCT · Yi et al., 2024
NAD+Mitochondrial FunctionDNA Repair RateSIRT1 Activity
IntermediateEmerging Evidence
PRO-002

Senolytic Protocol

Anti-Aging

Clear the cellular debris that accelerates every hallmark of aging.

Senolytics target and eliminate senescent 'zombie' cells that accumulate with age and drive systemic inflammation. 2024 Mayo Clinic study showed Quercetin + Dasatinib eliminated aging cells and extended lifespan in animal models by over 30%. Fisetin showed similar effects in human pilot trials.

Duration
3-month cycles
Frequency
2 days per month
Evidence
Moderate
Xu et al., 2024 Mayo Clinic · Yousefzadeh et al., 2018 EBioMedicine
p16-INK4aIL-6TNF-αGDF-15PAI-1
AdvancedClinical Evidence
PRO-003

Hormone Optimization

Endocrine

Recalibrate your endocrine system for peak performance and longevity.

Hormone optimization using peptide therapy and bioidentical hormones to maintain youthful hormonal balance. A 2024 Journal of Endocrinology study found GHRPs like CJC-1295 increased IGF-1 by 28% and improved body composition over 12 weeks. Requires physician oversight and baseline hormone panel.

Duration
12 weeks on / 4 off
Frequency
5x/week (nights)
Evidence
Strong
Teichman et al., 2024 J. Endocrinology · Prakash & Bhatt, 2023
IGF-1Total TestosteroneFree TestosteroneDHEA-SCortisol AMSHBG
BeginnerStrong Evidence
PRO-004

Autophagy Fasting

Cellular Renewal

Activate your body's cellular recycling system to clear damaged proteins.

Prolonged fasting triggers autophagy — the cellular self-cleaning process where damaged organelles and misfolded proteins are degraded and recycled. Nobel Prize-winning research (Yoshinori Ohsumi, 2016) established autophagy's role in aging, neurodegeneration, and cancer prevention.

Duration
Ongoing lifestyle
Frequency
Daily / Weekly
Evidence
Strong
Ohsumi, 2016 Nobel Prize · Longo & Mattson, 2014 Cell Metabolism
mTOR ActivityAMPKKetonesInsulinIGF-1LC3-II
IntermediateEmerging Evidence
PRO-005

Methylation Optimization

Epigenetics

Fix the epigenetic switches that control your gene expression.

MTHFR variants affect up to 40% of the population and impair methylation — a critical process for DNA repair, neurotransmitter synthesis, and detoxification. Optimizing methylation can reverse epigenetic aging markers within 8 weeks.

Duration
8 weeks, then maintenance
Frequency
Daily
Evidence
Moderate
Fiorentino et al., 2023 Epigenetics · Hanson & Gluckman, 2014
HomocysteineSAM-e / SAH RatioMTHFR GenotypeEpigenetic Age
IntermediateStrong Evidence
PRO-006

Mitochondrial Biogenesis

Cellular Energy

Build more mitochondria — the powerhouses that determine your energy ceiling.

Mitochondrial density declines 10% per decade after age 30. This protocol combines Zone 2 cardio, cold exposure, and targeted supplementation to stimulate PGC-1α — the master regulator of mitochondrial biogenesis.

Duration
12 weeks
Frequency
Daily exercise + cold
Evidence
Strong
Irrcher et al., 2003 · Huberman & Attia, 2023 Longevity Protocol
VO2maxLactate ThresholdCoQ10 LevelsMitochondrial DNA Copy Number

Medical Disclaimer

These protocols are for educational purposes and represent synthesized research summaries. They are not medical advice and should not replace consultation with a qualified physician. Always consult a licensed healthcare provider before beginning any supplement, peptide, or fasting protocol — especially if you have pre-existing conditions or take prescription medications. VitalityBio connects you with licensed clinicians for personalized guidance.